To include cell bioreactors in a LOV manifold, a loop
connects Port 3 of the LOV and the flow cell, as shown in
Fig. 5A, thus allowing an aliquot of beads to be delivered
through that port and captured in a microcolumn behind
the bead-retention plug–nozzle directly upstream
from the flow cell. Once the microcolumn is packed, it
can be perfused by the auxiliary syringe pump through
the loop with the resulting perfusate entering the flow
cell through the plug–nozzle. Inside the flow cell, the
perfusate is mixed with reagent for analyte detection
[36]. This manifold can be modified by connecting a
multiposition valve (MPV) to Port 2 and deleting the
loop connected to Port 3