We established easy,
fast and reliable tools using mRNA-based simplex PCR, multiplex
PCR and quantitative real-time PCR for the analysis of the
sequences from TnI, MRP and tropomodulin (Tmod) genes.
Messenger RNA-based RT-PCR methods utilize specific genes as
markers for the detection of meat adulteration. These methods also
facilitate the identification of the compositions of meat mixture
through specific gene amplification using quantitative real-time
PCR. We used the TnI gene as a target for the specific identification
of chicken, pork and goat, and the MRP gene was used to identify
beef. Moreover, we reported the first sequence of the Tmod gene
to identify ostrich in adulterated meat samples. Here, we described
the methods for the simultaneous and quantitative identification
of contaminating chicken, pork, goat, beef and ostrich meats, with
high sensitivity and specificity.
We established easy,fast and reliable tools using mRNA-based simplex PCR, multiplexPCR and quantitative real-time PCR for the analysis of thesequences from TnI, MRP and tropomodulin (Tmod) genes.Messenger RNA-based RT-PCR methods utilize specific genes asmarkers for the detection of meat adulteration. These methods alsofacilitate the identification of the compositions of meat mixturethrough specific gene amplification using quantitative real-timePCR. We used the TnI gene as a target for the specific identificationof chicken, pork and goat, and the MRP gene was used to identifybeef. Moreover, we reported the first sequence of the Tmod geneto identify ostrich in adulterated meat samples. Here, we describedthe methods for the simultaneous and quantitative identificationof contaminating chicken, pork, goat, beef and ostrich meats, withhigh sensitivity and specificity.
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