Searching for new microbial metabolites requires strategies directed at decreasing the probability of identifying known compounds. One approach involves the screening of actinomycetes, which have been less exploited in the past antimicrobial screening. Our studies revealed the presence of Bacillus sp. from the Himalayan soil. It also produced antibiotic against pathogenic microbes. Bacillus isolate produced metabolites and exhibited antimicrobial activities against a broad spectrum of bacteria [3]. Bacillus sp. has been described traditionally as aerobic saprophytic soil microorganism. However, our results showed ERIH-44 to be a Gram-positive bacterium, as reported widely for marine actinomycetes [10]. Valan Arasu et al. [20] reported in vitro antimicrobial activity of Streptomyces spp. ERI-3 isolated from Western ghats rock soil. Boudemagh et al. [4] reported isolation and molecular identification of Actinomycete microflora of some Saharian soils of southeast Algeria and tested for their antifungal activity.