Cultivation of the biological materials
Each isolated marine bacterium was cultured for 3 days in
100 ml of marine broth (Difco) in 500-ml Sakaguchi flasks
at 30°C on a rotary shaker at 100 rpm. The cells grown
from 2 l of the culture were collected by centrifugation, the
total yield of the cells being about 20–25 g.