Spraying of a low concentration (106
CFU/ml) of any of these BBLS isolates on
nonwounded citrus leaves resulted in no
lesions. In contrast, all isolates at a low
concentration caused lesions (Fig. 1C and
D) on citrus with needle-puncture inoculation;
however, the resulting lesions were of
varied sizes and required longer than a
month to develop. Wounding of citrus tissue
therefore appeared to be critical for lesion
development from low level inoculations.