Abstract
Conditions for the production and purification of α-amylase from a strain of Bacillus coagulans and a strain of Bacillus stearothermophilus, following growth at 35 and 55 °C. have been determined. The procedure followed for the purification of the enzyme preparations resulted in a 450–600-fold increase in enzyme activity.
A comparison of various properties of the amylase preparations showed that the only significant difference in the preparations produced at 35 °C. and those produced at 55 °C. was in the thermal stability at 90 °C. The 55 °C. preparations showed only a 6–10% reduction in activity after 1 hr., whereas the 35 °C. preparations showed a 90–92 % reduction in 1 hr.
1
Postdoctoral Research Fellow of the National Microbiological Institute, National Institutes of Health, 1952–1954. Present address: Dept. of Horticulture, Washington State College, Pullman, Washington.
2
The major part of this work was carried out in the Dept of Bacteriology, University of Texas, Austin, while the author was a Predoctoral Research Fellow of the National Institutes of Health.
Copyright © 1955 Published by Elsevier Inc.