At 21 and 42 d of age, blood samples were collected from the birds’ wing veins and allowed to clot in polyprolene tubes for 2 h at room temperature.The tubes were centrifuged at 1,000 × g, 4°C, for 10 min, and the supernatant was removed and tored at −20°C until analysis [22]. The antioxidant activities were measured by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free
radical scavenging assay [23] and a thiobarbituric acid reactive substances (TBARS) assay [24].