2.9. Thin layer chromatographyThe thin layer chromatography (TLC) was performed accord-ing to the method described in WHO guidelines (WHO, 1998) withsome modifications. Pre-prepared methanol extracts of leaf stemand flowers of A. oleraceae were dissolved in 10 mL of methanolseparately and 8 L of the solutions of each extract was spotted onTLC plates (Pre-coated silica gel 60 A 20 × 20 cm; 0.2 mm thickness)and dried. Mobile phase consisted of chloroform: dichloromethane:cyclohexane: methanol (5:4:1:0.4). Spots were detected under UV366 nm. Then plates were sprayed with Vanillin-Sulfuric acid anddried at 105◦C for 5 min.