Determination of ash: For the determination of ash,
clean empty crucible was placed in a muffle furnace at
600oC for an hour, cooled in desiccator and then weight
of empty crucible was noted (W ). One gram of each of 1
sample was taken in crucible (W2). The sample was
ignited over a burner with the help of blowpipe, until it is
charred. Then the crucible was placed in muffle furnace
at 550oC for 2-4 h. The appearances of gray white ash
indicate complete oxidation of all organic matter in the
sample. After ashing furnace was switch off. The
crucible was cooled and weighed (W3). Percent ash was
calculated by following formula: