Plantlets with well-developed roots were subsequently
transferred to hardening media. The hardening media
consisted of half strength MS media without any growth
regulators. After 25 days of incubation, the in vitro hardened
plantlets were transferred to different potting mixtures of soil :
vermicompost 2:1 (v/v), river-sand and soil : FYM 2:1(v/v)
maintained inside green house conditions of 22-24°C and
90-95 % RH.