Methanogens were inhibited with BESA in the second enrichment step at pH 7 with all pulp materials (Fig. S1). The hydrogen yields with dry birch, dry conifer, wet birch and wet conifer were
68 ± 6, 76 ± 4, 66 ± 10 and 68 ± 7 mL H2/g TS, respectively.
The hydrogen production yields were lower than in the first enrichment step, which may have been due to BESA inhibition of some Clostridia [17]. Acetate was the main soluble metabolite, while
propionate concentration was lower than in the first enrichment culture.
Methanogens were inhibited with BESA in the second enrichment step at pH 7 with all pulp materials (Fig. S1). The hydrogen yields with dry birch, dry conifer, wet birch and wet conifer were68 ± 6, 76 ± 4, 66 ± 10 and 68 ± 7 mL H2/g TS, respectively. The hydrogen production yields were lower than in the first enrichment step, which may have been due to BESA inhibition of some Clostridia [17]. Acetate was the main soluble metabolite, whilepropionate concentration was lower than in the first enrichment culture.
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