6.2 Place the other specimen in a Soxhlet extraction apparatus having a tared Soxhlet extraction flask. Set a small disk of
fine-mesh screen wire in the top of the crucible to prevent loss of specimen. Extract with 150 mL of ethanol-toluene solution for 6 to 8 h, keeping the liquid boiling briskly. This should provide four to six siphonings per hour.