Taxonomic identification of the selected yeast strain
The selected yeast strain was taxonomically identified by
26S rDNA sequencing and the assimilation ability of
sugars. The partial 26S rDNA of the strain was amplified
by PCR and directly sequenced based on a previous
method (Kurtzman & Robnett 1998). The homology of
the sequence was determined using the BLAST system
of the DNA Data Bank of Japan (DDBJ). The sugar assimilation
abilities were evaluated with an API 20C AUX
system (BioMerieux, Tokyo, Japan) according to the
manufacturer’s instructions.