Feed samples Perform two weightings for each sample. Two portions of pellets of about 50.0 g each, are weighed into 500 ml Erlenmeyer flasks. 500 ml acetate buffer with Tween 20, 4.5 (5.13) are added to each sample and the mixtures are stirred (600 700 rpm) for 1 hour with egg stirring bars ml extract are transferred to an Eppendorf tube (6.15) and centrifuged for 3 minutes The is transferred to a new tube and kept for 15-30 minutes on ice analysis (9.2,