1. Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
2. Open pouch of Botulism Toxin lateral flow device. Remove contents.
3. Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
4. Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
5. Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.