Pretreatment by physical, chemical or biological means is a well-investigated process for biofuel production from lignocellulosic materials. The pretreatment can enhance the bio-digestibility of the wastes for ethanol and biogas production and increase accessibility of the enzyme to the materials. It results in enrichment of the difficult biodegradable materials, and improves the yield of reducing sugar, ethanol or biogas from the wastes [13]. In present study, 1.71-folds of cellulose increased after pretreatment pineapple peel with water and heat at 100oC for 240 min as mention in Table 1. Pineapple peel treated with various physical and chemical pretreatment yield different sugars content. The highest level of reducing sugar (40.10±3.98 g/L) was observed from phosphoric acid treatment with water bath (100oC, 240 min), followed by hot water treatment (34.03±1.30 g/L) (Table 2). However, the phosphoric acid treatment was not selected for further study due to the fact that they are corrosive and must be recovered. Therefore, treatment with water in combination with water bath was selected to make the pretreatment economically feasible. In addition, the application of phosphoric acid caused the enzyme inhibitor after treatment process