2.5. Antagonistic activity of iturin A derivative peptides
Mycelial discs of Colletotrichum gloeosporioides, which causes
strawberry anthracnose and grape ripe rot diseases, were placed
on potato dextrose agar plates. At the same time, sterilized paper
discs were placed on the plates and then inoculated with 50 mL of
0.1 mg/ml iturin A or iturin A derivative peptides. The plates were
incubated at 25 C for 5 days. The antagonistic effects of the derivative
peptides toward C. gloeosporioides mycelial growth were
evaluated by measuring the growth inhibition zones formed on the
plates.
2.6. Statistics
Data are presented as means ± standard deviations. Statistical
analysis was performed by Tukey's test using Excel statistics software
2012 (Social Survey Research Information, Tokyo, Japan).