The obtained results are presented as mean values ± SD. The statistical calculations were made on the basis of analyzed with Excel 2003 (Microsoft) and Statistica version 6.0 (StafSoft Inc. Tulsa, OK, USA). The statistical significance of differences was evaluated using a One way and Two way analysis of variance (ANOVA). Mean values were compared by the Fisher's Least Significant Difference (LSD) Test or using Student's t-test. The differences were considered significant with P < 0.05.