2.6. Extracts of fermented red beans
Both water extract and ethanolic extract were prepared. One
gram of fermented red beans was suspended in 10 mL of dd H2O or
50% ethanol, and the mixture was stirred for 45 min. The precipitate
was removed by centrifugation at 8000 g for 10 min and the
supernatant was filter-sterilized (filter pore size 0.45 mm).