The detection
is mainly based on direct or indirect determination
of enzyme activity before and after the
application of the sample. The determination of
enzyme activity can be accomplished with different
enzyme substrates, which require different detection
methods. Most often applied are
potentiometric [5–9], amperometric [10–20], optical
[21–25] and conductometric [26] detectors.
Biorecognition based techniques result in rapid,
simple and selective methods for pesticide analysis
because they combine the selectivity of the enzymatic
reactions with operational simplicity. Recently
a new detection method (thermal lens
spectrometry) was applied in combination with a
cholinesterase biosensor, showing low limits of
detection [27].