To investigate the influence of temperature on the formation of fungal biomass and exopolysaccharides, the cultures of G. applanatum and T. palustris were carried out at various temperatures in the range from 20 to 37 °C for 10 and 7 days, respectively (the optimal culture time was experimentally confirmed in earlier investigations, data not
shown). As shown in Fig. 2, both the maximum biomass
formation and the exopolysaccharide production during
cultivation of G. applanatum were observed during the
growth at 28 °C, with the dry weight of biomass around
3.64 ± 0.2 g/L and exopolysaccharide yield reaching
30.78 ± 0.6 g/L. The optimal temperature for biomass
formation during cultivation of G. applanatum corresponded with that for the production of exopolysaccharides
and for the secretion of oxalic acid. At the same temperature, we also noticed the maximum level of oxalic acid
(1.24 ± 0.2 mM) in the culture medium of G. applanatum.
Apart from oxalic acid, the presence of succinic acid and
tartaric acid was observed during the growth of G. applanatum, but these two acids were detected only
in trace
amounts (Fig. 3). The highest concentration of succinic
acid (0.7 ± 0.01 mM) and tartaric acid (3.55 ± 0.03 mM)
was observed only on the 11th day of cultivation of G.
applanatum at 37 °C. T. palustris grew optimally in all the
tested temperatures and the maximum dry weight of
biomass was obtained at 37 °C (0.38 ± 0.02 g/L), while
the maximum amount of exopolysaccharides was observed
during the growth at a temperature of 20 °C (19.1 ± 1.2 g/
L). It was observed that the concentration of oxalic acid in
the culture media of T. palustris increased when the culture
temperature increased from 20 to 37 °C, and the highest
amount of oxalic acid was obtained in the cultures growing
at 37 °C (21.1 ± 1.1 mM). The results indicate that the
optimal temperature for the cell growth of T. palustris is
not consistent with the optimal temperature for the secre-
tion of oxalic acid.
To investigate the influence of temperature on the formation of fungal biomass and exopolysaccharides, the cultures of G. applanatum and T. palustris were carried out at various temperatures in the range from 20 to 37 °C for 10 and 7 days, respectively (the optimal culture time was experimentally confirmed in earlier investigations, data notshown). As shown in Fig. 2, both the maximum biomassformation and the exopolysaccharide production duringcultivation of G. applanatum were observed during thegrowth at 28 °C, with the dry weight of biomass around3.64 ± 0.2 g/L and exopolysaccharide yield reaching30.78 ± 0.6 g/L. The optimal temperature for biomassformation during cultivation of G. applanatum corresponded with that for the production of exopolysaccharidesand for the secretion of oxalic acid. At the same temperature, we also noticed the maximum level of oxalic acid(1.24 ± 0.2 mM) in the culture medium of G. applanatum.Apart from oxalic acid, the presence of succinic acid andtartaric acid was observed during the growth of G. applanatum, but these two acids were detected only in traceamounts (Fig. 3). The highest concentration of succinicacid (0.7 ± 0.01 mM) and tartaric acid (3.55 ± 0.03 mM)was observed only on the 11th day of cultivation of G.applanatum at 37 °C. T. palustris grew optimally in all thetested temperatures and the maximum dry weight ofbiomass was obtained at 37 °C (0.38 ± 0.02 g/L), whilethe maximum amount of exopolysaccharides was observed
during the growth at a temperature of 20 °C (19.1 ± 1.2 g/
L). It was observed that the concentration of oxalic acid in
the culture media of T. palustris increased when the culture
temperature increased from 20 to 37 °C, and the highest
amount of oxalic acid was obtained in the cultures growing
at 37 °C (21.1 ± 1.1 mM). The results indicate that the
optimal temperature for the cell growth of T. palustris is
not consistent with the optimal temperature for the secre-
tion of oxalic acid.
การแปล กรุณารอสักครู่..