2.5.2. Determination of total phenol (TPC) and flavonoid contents (TFC)
Total phenol content (TPC) of chocolate extracts was determined
spectrophotometrically by the Folin–Ciocalteu reagent, according to a
modified method of Lachman, Hosnedl, Pivec, and Orsák (1998). The
total polyphenol colorimetric assaywas initially developed as a method
for the analysis of proteins, but was adapted later by Singleton to measure
phenolic compounds in wine (Singleton & Rossi, 1965). Although
the application of F–C reagent actually measures a sample's reducing