Molecular mass distribution of protein ingredients was determined using a modification of the method of O'Loughlin, Murray, Kelly, Fitzgerald, and Brodkorb (2012).
Size-exclusion chromatography was carried out using TSK Gel G2000SWxL and G3000SWxL(7.8 mm 300 mm) columns, operated in series (TosoHaas Bioscience GmbH, Stuttgart, Germany), under isocratic conditions (20 mM sodium phosphate buffer, pH 7) over 60 min and at a flow
rate of 0.5 mL min1