2.5. Antioxidant activity (AOA) determination
The antioxidant activity (AOA) of extracts was determined by
auto oxidation of a ß-carotene and linoleic acid coupled reaction,
according to Emmons and Peterson (1999). ß-carotene (6 mg)
was dissolved in 20 ml of chloroform. A 3 ml of the solution was
added to a conical flask with 40 ll linoleic acid and 400 ll Tween
20.