For HPLC analysis, a waters symmetry C18 column (4.6 x 250 mm 5m) was used with a linear gradient of Buffer: methanol (96:4) at a constant flow rate of 1 ml/min with 2300 pressure by using waters pump (1515 isocratic) and a UV (2487) detector was employed for the detection of peaks, using two channels simultaneously at a wavelength of 210 nm, a bandwidth of 5 nm and another wave-length of 280 nm.