These inoculated tubes
were then incubated at 44.5 ºC ± 0.2ºC for 24 hours in a water bath
(banho-maria). Should there be confirmation of Escherichia coli then
the positive tubes in the EC broth were striated in boards containing
Methylene Blue Eosin Agaro (EMB). After incubation at 35 ºC for 24
hours, five typical colonies of E. coli (dark and smooth colonies withor without metallic shine) were transferred to incline nutritional Agar