in the original study of Beaz-Hidalgo et al. (2008) that proposed these PCR methods, the sensitivity and specificity was evaluated with 69 biochemically identified A. salmonicida strains including only two of the five subspecies (achromogenes and masoucida) and 38 strains belonging to other bacteria implicated in fish disease.
The latter included several Aeromonas spp. i.e. Aeromonas hydrophila (n = 12), A. bestiarum (n = 1), Aeromonas caviae (n = 1), A. media (n = 1) and A. sobria (n = 1)