3. Results
3.1. Sample treatment
3.1.1. Acid digestion as sample preparation
Based on reported procedures for bone, pig kidney and bovine
liver a unique sample treatment was evaluated for the different
tissues of interest.
For bone samples, the literature suggests masses between
0.1 and 1 g of dried sample, which can be mineralized with nitric
acid volumes varying from 5 to 10 mL [11–14]. These mixtures
were heated in a microwave oven, using closed vessels and
heating programs demanding 15 or 40 min [11–14].
For liver and kidney samples the procedures used are similar:
masses from 0.02 and 0.50 g of dried sample were mixed with
concentrated nitric acid [15–17] and hydrogen peroxide [18]. The
volumes of nitric acid varied from 0.4 to 7 mL, while those for