Biomass sample was prepared based on the established procedures
of our previous work [32]. Cultivation of Y. lipolytica Po1g
cells was performed in 500 mL conical flasks each containing
250 mL culture media in an orbital shaker incubator model LM-570
(Chemist Scientific Co., Taiwan) at 26 °C and 160 rpm. The biomass
was collected, separated from the aqueous phase by centrifugation
and then subjected to freeze drying (Freeze Zone — 2.5 L freeze dry
system-Model 7670520, Labconco Corporation, Kansas City, USA).
The freeze-dried biomass sample was then collected and stored at
4 °C before use.