3.3. Reproducibility
The intra-assay reproducibility was analyzed by using 10-fold dilutions of the plasmid DNA standard ranging from 2.88 × 107 to 2.88 × 100copies/l in triplicates per run. The calculated standard deviation (SD) and coefficient of variation (CV) values were, rangingfrom 0.05 to 0.56 and from 0.21 to 1.83%, respectively. The inter-assay CV and SD were in the range of 0.44 to 1.97% and 0.08 to 0.61,respectively.