2.3.2. Preparation of CCWP(2)
Aqueous ethanol (70%) (500 ml) was added to defatted VOR (100 g) and stirred for 1 h. The contents were filtered through double layers of a linen cloth and washed with distilled water to isolate aqueous ethanol insoluble cell wall polysaccharides. The aqueous ethanol insoluble matter was re-suspended in 70% ethanol (1:5 w/v) and the above procedure was repeated two times. After the third wash, aqueous ethanol insoluble matter was collected by drying at 70 °C to obtain CCWP(2).