The objective of this study was to evaluate the effects of Lactobacillus kefiri (strain DSM
19455) and Lactobacillus brevis (strain DSM 23231) on the fermentation and aerobic stability
of sugarcane silage. After chopping, sugarcane was treated as follows (as fed basis): no
additives (C), Lactobacillus brevis DSM 23231 at 2 × 105 cfu/g (Lb), Lactobacillus kefiri DSM
19455 at 2 × 105 cfu/g (Lk), Lactobacillus brevis DSM 23231 at 1 × 105 cfu/g plus Lactobacillus
kefiri DSM 19455 at 1 × 105 cfu/g (Lb + Lk). Treated forage was packed in laboratory silos
(capacity 20 L) and stored for 75 d. All inoculants decreased nutrientlosses during anaerobic
storage, by atleast 26%. Inoculated silages (Lb, Lk, and Lb + Lk) had higher soluble sugars and
lower neutral detergent fiber (NDFom) concentrations. Silage inoculated with Lk had higher
in vitro true DM digestibility than the control and Lb treatments. Although all silages had
low pH values (≤3.86), the inoculants decreased the concentrations of ethanol, lactic acid,
and most trace compounds. Treatment with Lk increased the concentration of acetic acid
and 1,2-propanediol and decreased the yeast population compared with that of all other
treatments. Compared with untreated silage, inoculation was unable to improve stability
when silages were exposed to air. In conclusion, L. kefiri and L. brevis applied at 2 × 105 cfu/g
were able to mitigate the formation of volatile organic compounds and decreased the loss
of nutrients during the fermentation of sugarcane silages