Polysaccharide concentrations in water were determined using the phenol-sulfuric acid method with glucose as the standard. Significant differences in polysaccharide rejection for different feed waters by UF were analyzed by SPSS (SPSS, USA) software. The absorbance at 490 nm was measured, and the polysaccharide concentration was expressed as glucose equivalent. Protein concentrations in water were determined using three-dimensional fluorescence excitation-emission matrix (3D-EEM) spectra (F-7000, Hitachi, Japan). The excitation wavelength (Ex) range was between 220 and 450 nm, and the emission wavelength (Em) was between 240 and 600 nm. The sampling interval was 5 nm, the response time was 0.01 sec and the scanning speed was 2400 nm/min. The spectral data were analyzed using Origin (OriginLab, USA) software. Spectral subtraction was performed to remove blank spectra that were mainly caused by Raman scattering