In the cross-reactivity study, no positive band was detected in WB antigens from five trematode parasites (G. explanatum, E. pancreaticum, P. cervi, S. spindale and S. mansoni) and from two nematode parasites (Haemonchus placei and S. labiato-papillosa), while the band was very prominent in WB of F. gigantica NEJ (Fig. 3).