The alignment of nanofibers was assesses by measuring the angle of fiber from SEM images (n = 50 at each condition); The degree of alignment was determined as a reference angle (zero angle) and all deviations were measured relative to that angle. these values were then normalized to 90 degrees and plotted in a histogram.Closer to 90 degrees indicates more alignment. The histogram illustrates the alignment of fiber distribution as a function of frequency (number of fibers that lie in the alignment of fiber group).