2.3.4. Acclimation
In this study, the culture water and media used in the stock cultures of the test organisms were the same as those used in the solutions for the toxicity tests. In addition, the culture conditions (temperature, light intensity, and light–dark periods) applied to the stock cultures were similar to those applied in the toxicity tests. Therefore, the organisms in the stock cultures were considered to be acclimated and were used directly in the tests