Glucose clamping is perhaps the flavoured technique because it gives a clear numerical value and allows construction of dose-response curves. An infusion of glucose is produce logarithmically increasing circulating insulin concentration of say 100, 1,000 and 10,000 mU/L. the combination of insulin infusion to a circulating concentration of 100 mU/L with glucose infusion effectively block hepatic glucose output . Under these steady-state conditions, the amount of glucose infusion to maintain blood glucose concentration must equal the amount that is being taken up by peripheral tissue, thus giving an indirect measure of glucose disposal. Clearly. The lower the amount of glucose uptake by the tissue, the greater is the insulin resistance.