The fragment p40-internal ribosome entry
site-p35 was inserted into the El deleted adenovirus backbone
pAd.l/Rous sarcoma virus (6). The recombinant adenovirus,
ADV/mIL-12, was generated by cotransfection
with pBHG10 (7) into 293 cells. The viral titer [plaqueforming
units (pfu)/ml] was determined by plaque assay in
293 cells (8).
Functional Analysis of