Coleonema album is an aromatic, medicinal and ornamental plant that is in high demand in the traditional medicine
market. In order tomeet the increasing demand while conserving those populations in the wild, this study
was aimed at developing an efficient in vitro propagation protocol for C. album. The effect of different cytokinins
[four meta-topolins in comparison with 6-benzyladenine (BA), kinetin (KIN) and thidiazuron (TDZ)] on direct
organogenesis from shoot-tip explants was examined. Each cytokinin was tested at 2.5, 5.0, 7.5 and 10.0 μM.
The effect of combining auxins with the optimal cytokinin concentration on shoot proliferation was also investigated.
At equimolar concentrations, all treatments with BA, meta-topolin (mT, except 10 μM), meta-topolin
riboside and meta-methoxytopolin tetrahydropyran-2-yl significantly increased shoot production when compared
to KIN. The optimumcytokinin concentration for shoot productionwas 5.0 μMmTwith 14.5 shoots regenerated
per explant. The exogenous addition of auxins further increased shoot proliferation (up to 18 shoots per
explant). The findings suggest an additive or synergistic effect of auxinwithmT in this study. Regenerated shoots
rooted on half-strengthMurashige and Skoog mediumsupplementedwith indole-3-butyric acid (IBA)were successfully
acclimatized with a survival frequency above 70%.