This work aimed to evaluate the tomato (Lycopersicon esculentum Mill.) seed quality obtained from fruits
harvested at different trusses of plant. In each truss, fruits were harvested at proximal and distal positions, at 70
DAA. Fruit weight, seed yield per fruit, seed moisture and dry matter content, standard germination, germination
at first count, germination after controlled deterioration, seedling emergence percentage, speed emergence index
and electrical conductivity were determined. Fruit weight decreased throughout fructification, from the first to
the sixth truss, but this decrease was not reflected in reduced seed quality, because, in general high vigor seeds
were obtained from the late trusses. The seeds extracted from fruits of the fourth to sixth trusses had, overall,
better quality than those from the first to third truss. On the same truss, the distal fruits tended to produce seeds
of higher physiological quality than the proximal fruits.