Two culture media were employed in the present study: YPS
proliferation medium (yeast extract 10 g/L, peptone 10 g/L and
sucrose 50 g/L) for reactivation and propagation of yeasts; and YPS
fermentation medium (yeast extract 10 g/L, peptone 10 g/L and
sucrose 250 g/L) for fermentation [11]. Additionally, sugarcane
molasses was evaluated after collecting sugarcane molasses from
local sugarcane mills (Tucum an, Argentina) and diluted to achieve
25% of total reducing sugars (TRS). All these media were sterilized
in autoclave at 121
C during 15 min. Agarized YPS proliferation
medium, used to isolate yeasts, was prepared with YPS proliferation medium and 15 g/L agar