Antimicrobial films were produced from wheat proteins according to the method described by Balaguer, Gomez-Estaca, Gavara and Hernandez-Munoz [9]. Basically, a solution rich in gliadins was extracted from wheat gluten and different percentages of the cross-linker, namely 1.5% (G1.5C_pH2), 3% (G3C_pH2), and 5% (G5C_pH2) (g cinnamaldehyde/100 g protein) were incorporated into the solution. Glycerol was added as a plasticizer at 25% (g/100 g protein). The film-forming solution containing the plasticizer and different amounts of the cross-linker was adjusted at pH 2 with HCl and stirred for 30 min. Films were produced by casting and subsequent evaporation of the solvent at 37 C for 24 h. A gliadin film produced at the native pH of the gliadin filmforming solution (pH 6) and without cinnamaldehyde addition was employed as the control (G_pH6). The mean film thickness was 100 ± 12 lm measured using a micrometer (Mitutoyo, Kanagawa, Japan), and calculated from measurements taken at ten different locations on each film sample. The grammage of the films was 0.015 ± 0.002 g/cm2 measured at 50% RH (relative humidity) and 23 C. Actual concentration of cinnamaldehyde after the production of the films was evaluated in a previous work by solvent extraction followed by gas chromatography determination.Remaining cinnamaldehyde was present in concentrations lower than 1% [12]; therefore,biodegradation of this constituent was not necessary to be tested separately.