Similarly,
extract from chrysene-grown cultures were also derivatized
and subjected to test for the presence of these six compounds. After
the vacuum drying of each eluate from chrysene-grown cultures
(100l) in a vial, N,O-bis-trimethylsilyl acetamide (40l), pyridine
(40l), and trimethylchlorosilane (20l)were added. Trimethylsilylation
of the eluate was conducted for 10 min at 80 ◦C without
contact with moisture.