The reaction mixture was
incubated at 50 C for 60 min. Endoglucanase activity was
carried out by using 0.1 mL of suitably diluted enzyme and
0.9 mL of 40 g L1 carboxymethylcellulose (CMC) (Sigma, St.
Louis, EUA) solution in citrate buffer (50mmol L1, pH 5.0).