with monthly transfers. The 24-hour cultures developed on TSA at 30–35°C were harvested in a tryptic soy broth (TSB, Difco), centrifuged (1000 g/ 15 min/ 4°C), and suspended in saline (0.95% NaCl plus 0.1% peptone) to a final population (by pour plate) of 106 CFU/mL, and the suspensions were applied to the MIC tests.