Seeds were then examined with an UV-fluorescence microscope (LSM710 ImagerZ2, Zeiss, Germany) for assessing the condition of the testa and embryo cell viability
The fresh seeds were also used directly without any pretreatment to obtain micrographs (Fig. 1(d)) using a scanning electron microscope (EVO LS10, Zeiss, Germany). Further experiments on the harvested seeds were conducted in tissue culture rooms under sterile conditions at 25 ± 2.0 °C and at 45% relative humidity (RH).