At fermentation days 1 and 3, the pH in sample S500 was significantly lower than of S250 and S125. However, after 6 days of fermentation, therewas no significant difference among all samples studied. At day 0, the titratable acidity in all samples ranged between 0.05 and 0.07%. After 6 days of fermentation, the titratable acidity among all samples increased to 0.29e0.34%, indicating that the difference in mushroom concentration did not result in significant variations of broth pH.