Determination of total protein: Five gram of grinded mushroom was taken with 50ml of
1N NaOH and boiled for 30 minutes. The solution was cooled in room temperature and
centrifuged at 1000 × g by a table centrifuge machine (DIGISYSTEM: DSC-200T;
Taiwan). The supernatant was collected and total protein content was measured according
to the Biuret method (Burtis & Ashwood, 2006).