A polyhydroxyalkanoate (PHA) producing Gram-positive, rod-shaped, motile
bacterium was isolated from the polluted pond water. Strain SKM-11 grew at 10–
40oC and pH 5.0–8.0 and in the presence of 0–3 % (w/v) NaCl. Antimicrobial
activities were studied. The DNA G+C content was 55mol%. Phylogenetic analysis
based on 16S rRNA gene sequences showed that strain is a member of the genus
Bacillus and is most closely related to B. aryabhattai B8W22T
(99.66%), B.
megaterium IAMB418T (99.25%), B. flexus IF015715T (98.34%). The sequence of
the 16SrDNA gene of strain SKM-11 was determined was 1466bp and deposited in
the EMBL under accession no. LM655313. The phenotypic and genotypic
properties clearly indicate that strain represents a novel species of the genus
Bacillus. The strain produced PHA in mineral medium consisting of glucose and
nitrogenous substances. Optimum conditions for polymer production were
determined, extracted and confirmed through Spectroscopic. Starin SKM11=
KCTC3368T
.