2.4. Characterization of cell growth and PHB production of the selected
strains
The Pre-culture was prepared by cultivating a single colony of
Bacillus megaterium R11 in LB liquid medium at 30 C for 24 h. Cells
were then collected by centrifuge at 8000g for 7 min, re-suspended
in sterile 9 g/L NaCl solution to OD600 of 10 and stored at
4 C until use.